Polymorphism and Association of DMA Gene with Total IgY Concentration and ND Antibody Titer in IPB-D2 Chicken Line

The DMA (DM ? chain) gene, a member of non-classical MHC class II plays an important role in the process presenting antigen peptides by producing DM protein. This study aimed to identify polymorphism gene and their associations with disease resistance traits such as total IgY concentration ND antibody titers IPB-D2 chicken line. sample used was 101 chickens consisted 81 G0 (53 female 28 male) 20 SENSI-1 (15 5 male). Blood samples were collected at 21 weeks old for genotyping, analysis, titer analysis. comparison analysis polymorphism. method DNA direct-DNA sequencing. analyzed using indirect ELISA method, while HI test. Data GLM, genotypic, haplotype mean values compared t-test. results showed that 4 SNPs found, i.e., g.2328 G>A (exon 3), g.2503 A>G (intron g.2612 G>A, g.2686 4). found polymorphic three genotypes (GG, AG, GG), expected SNP only had two (AA GG). associated (p<0.05) concentration. A combination formed 7 haplotypes. Haplotype 1 (p<0.05), (p<0.05). In conclusion, mutation could be potentially recommended genetic marker high concentration,